ParE

• Description: subunit of DNA topoisomerase IV
  
  

Gene name	parE
Synonyms	grlB
Essential	yes PubMed
Product	subunit of DNA topoisomerase IV
Function	chromosome segregation and compaction
Gene expression levels in SubtiExpress: parE
MW, pI	72 kDa, 6.166
Gene length, protein length	1965 bp, 655 aa
Immediate neighbours	yneT, parC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA condensation/ segregation, essential genes

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

• Locus tag: BSU18090

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU18090

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: type II topoisomerase family (according to Swiss-Prot)

• Paralogous protein(s): GyrB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • Nucleoid (Homogeneous) PubMed
  • uniformly distributed throughout the cell PubMed

Database entries

• BsubCyc: BSU18090

• Structure:

• UniProt: Q59192

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: parE-parC PubMed

• Expression browser: parE PubMed

• Sigma factor:

• Regulation:
  • induced by DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Serkalem Tadesse, Judita Mascarenhas, Bernd Kösters, Andrej Hasilik, Peter L Graumann
Genetic interaction of the SMC complex with topoisomerase IV in Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 11);3729-3737
[PubMed:16272394] [WorldCat.org] [DOI] (P p)

Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290] [WorldCat.org] [DOI] (P p)

W M Huang, J L Libbey, P van der Hoeven, S X Yu
Bipolar localization of Bacillus subtilis topoisomerase IV, an enzyme required for chromosome segregation.
Proc Natl Acad Sci U S A: 1998, 95(8);4652-7
[PubMed:9539793] [WorldCat.org] [DOI] (P p)