PbuE

• Description: putative hypoxanthine exporter
  
  

Gene name	pbuE
Synonyms	ydhL
Essential	no
Product	putative hypoxanthine exporter
Function	nucleotide metabolism
Gene expression levels in SubtiExpress: pbuE
MW, pI	41 kDa, 8.884
Gene length, protein length	1164 bp, 388 aa
Immediate neighbours	ydhK, gmuB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, nucleotide metabolism/ other, membrane proteins

This gene is a member of the following regulons

A-box

The gene

Basic information

• Locus tag: BSU05800

Database entries

• BsubCyc: BSU05800

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU05800

• Structure:

• UniProt: Q797E3

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: pbuE PubMed

• Sigma factor:

• Regulation:
  • induced by hypoxanthine and guanine, PurR-independent PubMed
  • induced by adenine (A-box) PubMed

• Regulatory mechanism:
  • A-box: adenine-controlled riboswitch, transcription termination/ antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jean-François Lemay, Guillaume Desnoyers, Simon Blouin, Benoit Heppell, Laurène Bastet, Patrick St-Pierre, Eric Massé, Daniel A Lafontaine
Comparative study between transcriptionally- and translationally-acting adenine riboswitches reveals key differences in riboswitch regulatory mechanisms.
PLoS Genet: 2011, 7(1);e1001278
[PubMed:21283784] [WorldCat.org] [DOI] (I e)

Vanessa Delfosse, Patricia Bouchard, Eric Bonneau, Pierre Dagenais, Jean-François Lemay, Daniel A Lafontaine, Pascale Legault
Riboswitch structure: an internal residue mimicking the purine ligand.
Nucleic Acids Res: 2010, 38(6);2057-68
[PubMed:20022916] [WorldCat.org] [DOI] (I p)

Elias Seif, Sidney Altman
RNase P cleaves the adenine riboswitch and stabilizes pbuE mRNA in Bacillus subtilis.
RNA: 2008, 14(6);1237-43
[PubMed:18441052] [WorldCat.org] [DOI] (I p)

Natalia P Zakataeva, Sergey V Gronskiy, Anastasia S Sheremet, Ekaterina A Kutukova, Anna E Novikova, Vitaliy A Livshits
A new function for the Bacillus PbuE purine base efflux pump: efflux of purine nucleosides.
Res Microbiol: 2007, 158(8-9);659-65
[PubMed:17935948] [WorldCat.org] [DOI] (P p)

K V Lobanov, N V Korol'kova, S Iu Eremina, L Errais Lopes, S A Proshkin, A S Mironov
[Mutations altering the specificity of the sensor RNA encoded by the Bacillus subtilis pbuE gene].
Genetika: 2007, 43(6);859-64
[PubMed:17853814] [WorldCat.org] (P p)

Jean-François Lemay, J Carlos Penedo, Renaud Tremblay, David M J Lilley, Daniel A Lafontaine
Folding of the adenine riboswitch.
Chem Biol: 2006, 13(8);857-68
[PubMed:16931335] [WorldCat.org] [DOI] (P p)

J Kenneth Wickiser, Ming T Cheah, Ronald R Breaker, Donald M Crothers
The kinetics of ligand binding by an adenine-sensing riboswitch.
Biochemistry: 2005, 44(40);13404-14
[PubMed:16201765] [WorldCat.org] [DOI] (P p)

Per Nygaard, Hans H Saxild
The purine efflux pump PbuE in Bacillus subtilis modulates expression of the PurR and G-box (XptR) regulons by adjusting the purine base pool size.
J Bacteriol: 2005, 187(2);791-4
[PubMed:15629952] [WorldCat.org] [DOI] (P p)

Maumita Mandal, Ronald R Breaker
Adenine riboswitches and gene activation by disruption of a transcription terminator.
Nat Struct Mol Biol: 2004, 11(1);29-35
[PubMed:14718920] [WorldCat.org] [DOI] (P p)

Lars Engholm Johansen, Per Nygaard, Catharina Lassen, Yvonne Agersø, Hans H Saxild
Definition of a second Bacillus subtilis pur regulon comprising the pur and xpt-pbuX operons plus pbuG, nupG (yxjA), and pbuE (ydhL).
J Bacteriol: 2003, 185(17);5200-9
[PubMed:12923093] [WorldCat.org] [DOI] (P p)

Maumita Mandal, Benjamin Boese, Jeffrey E Barrick, Wade C Winkler, Ronald R Breaker
Riboswitches control fundamental biochemical pathways in Bacillus subtilis and other bacteria.
Cell: 2003, 113(5);577-86
[PubMed:12787499] [WorldCat.org] [DOI] (P p)