ydfJ

168

phospholipid efflux pump

Locus: BSU_05430

Molecular weight: 76.85 kDa

Isoelectric point: 5.18

Protein length: 724 aa

Gene length: 2175 bp

Function: resistance to the membrane-acting antibiotic rhodomyrtone

Product: phospholipid efflux pump

Essential: no

E.C.: null

Synonyms: ydfJ, farE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2409 (Galperin et al., 2021)

Gene

Coordinates: 589,717  591,891

The protein

Catalyzed reaction/ biological activity

• export of phosphatidylglycerol-containing phospholipids

Protein family

• resistance-nodulation-cell division (RND) (TC 2.A.6) family (with YdgH and SwrC, according to UniProt)

Structure

• 6N3T (PDB) (from Mycobacterium smegmatis, 25% identity) PubMed
• P96687 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: ydfJ

  Description: PubMed

  Regulatory mechanism

  • YdfI: activation, PubMed, in ydfI regulon

Biological materials

Mutant

• MGNA-C149 (ydfJ::erm), available at the NBRP B. subtilis, Japan
• BKE05430 (ydfJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATAATCTCCTTTCAAC,  downstream forward: _UP4_TAATAGAAAAAGCAGATCTT
• BKK05430 (ydfJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATAATCTCCTTTCAAC,  downstream forward: _UP4_TAATAGAAAAAGCAGATCTT

References

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