HutI

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  • Description: imidazolone-5-propionate hydrolase

Gene name hutI
Synonyms
Essential no
Product imidazolone-5-propionate hydrolase
Function histidine utilization
Gene expression levels in SubtiExpress: hutI
Metabolic function and regulation of this protein in SubtiPathways:
His
MW, pI 45 kDa, 5.215
Gene length, protein length 1263 bp, 421 aa
Immediate neighbours hutU, hutG
Sequences Protein DNA DNA_with_flanks
Genetic context
HutI context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
HutI expression.png



















Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

CcpA regulon, CodY regulon, HutP regulon

The gene

Basic information

  • Locus tag: BSU39370

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+ (according to Swiss-Prot)
  • Protein family: hutI family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed by glucose (CcpA) PubMed
    • induced by histidine (HutP) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Robert A Bender
Regulation of the histidine utilization (hut) system in bacteria.
Microbiol Mol Biol Rev: 2012, 76(3);565-84
[PubMed:22933560] [WorldCat.org] [DOI] (I p)


Original publications