PcrB

• Description: heptaprenylglyceryl phosphate synthase
  
  

Gene name	pcrB
Synonyms	yerE
Essential	no
Product	heptaprenylglyceryl phosphate synthase
Function	unknown
Gene expression levels in SubtiExpress: pcrB
MW, pI	24 kDa, 4.204
Gene length, protein length	684 bp, 228 aa
Immediate neighbours	yerD, pcrA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

lipid metabolism/ other

The gene

Basic information

• Locus tag: BSU06600

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • production of heptaprenylglyceryl phosphate from heptaprenyl diphosphate and glycerol-1-phosphate PubMed

• Protein family: GGGP synthase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • forms dimers PubMed

• Localization:

Database entries

• Structure: 1VIZ PubMed

• UniProt: O34790

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pcrB-pcrA-ligA-yerH PubMed

• Expression browser: pcrB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Feifei Ren, Xinxin Feng, Tzu-Ping Ko, Chun-Hsiang Huang, Yumei Hu, Hsiu-Chien Chan, Yi-Liang Liu, Ke Wang, Chun-Chi Chen, Xuefei Pang, Miao He, Yujie Li, Eric Oldfield, Rey-Ting Guo
Insights into TIM-barrel prenyl transferase mechanisms: crystal structures of PcrB from Bacillus subtilis and Staphylococcus aureus.
Chembiochem: 2013, 14(2);195-9
[PubMed:23322418] [WorldCat.org] [DOI] (I p)

David Peterhoff, Hermann Zellner, Harald Guldan, Rainer Merkl, Reinhard Sterner, Patrick Babinger
Dimerization determines substrate specificity of a bacterial prenyltransferase.
Chembiochem: 2012, 13(9);1297-303
[PubMed:22614947] [WorldCat.org] [DOI] (I p)

Harald Guldan, Frank-Michael Matysik, Marco Bocola, Reinhard Sterner, Patrick Babinger
Functional assignment of an enzyme that catalyzes the synthesis of an archaea-type ether lipid in bacteria.
Angew Chem Int Ed Engl: 2011, 50(35);8188-91
[PubMed:21761520] [WorldCat.org] [DOI] (I p)

Harald Guldan, Reinhard Sterner, Patrick Babinger
Identification and characterization of a bacterial glycerol-1-phosphate dehydrogenase: Ni(2+)-dependent AraM from Bacillus subtilis.
Biochemistry: 2008, 47(28);7376-84
[PubMed:18558723] [WorldCat.org] [DOI] (I p)

M A Petit, E Dervyn, M Rose, K D Entian, S McGovern, S D Ehrlich, C Bruand
PcrA is an essential DNA helicase of Bacillus subtilis fulfilling functions both in repair and rolling-circle replication.
Mol Microbiol: 1998, 29(1);261-73
[PubMed:9701819] [WorldCat.org] [DOI] (P p)