GutB

• Description: D-sorbitol dehydrogenase
  
  

Gene name	gutB
Synonyms
Essential	no
Product	D-sorbitol dehydrogenase
Function	glucitol utilization
Gene expression levels in SubtiExpress: gutB
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	38 kDa, 5.213
Gene length, protein length	1059 bp, 353 aa
Immediate neighbours	gutR, gutP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

GutR regulon

The gene

Basic information

• Locus tag: BSU06150

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-iditol + NAD⁺ = L-sorbose + NADH (according to Swiss-Prot)

• Protein family: zinc-containing alcohol dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: Q06004

• KEGG entry: [3]

• E.C. number: 1.1.1.14

Expression and regulation

• Operon: gutB-gutP PubMed

• Expression browser: gutB PubMed

• Sigma factor: SigA PubMed

• Regulation: induced by glucitol (GutR) PubMed

• Regulatory mechanism: GutR: transcriptional activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Shouji Watanabe, Miyuki Hamano, Hiroshi Kakeshita, Keigo Bunai, Shigeo Tojo, Hirotake Yamaguchi, Yasutaro Fujita, Sui-Lam Wong, Kunio Yamane
Mannitol-1-phosphate dehydrogenase (MtlD) is required for mannitol and glucitol assimilation in Bacillus subtilis: possible cooperation of mtl and gut operons.
J Bacteriol: 2003, 185(16);4816-24
[PubMed:12897001] [WorldCat.org] [DOI] (P p)

R Ye, S L Wong
Transcriptional regulation of the Bacillus subtilis glucitol dehydrogenase gene.
J Bacteriol: 1994, 176(11);3314-20
[PubMed:8195086] [WorldCat.org] [DOI] (P p)

K Ng, R Ye, X C Wu, S L Wong
Sorbitol dehydrogenase from Bacillus subtilis. Purification, characterization, and gene cloning.
J Biol Chem: 1992, 267(35);24989-94
[PubMed:1460002] [WorldCat.org] (P p)