TsaC

• Description: L-threonylcarbamoyl-AMP synthase, biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A) (together with TsaB-TsaD-TsaE)
  
  

Gene name	tsaC
Synonyms	ipc-29d, ywlC
Essential	no
Product	L-threonylcarbamoyl-AMP synthase
Function	tRNA modification
Gene expression levels in SubtiExpress: tsaC
MW, pI	36 kDa, 5.82
Gene length, protein length	1038 bp, 346 aa
Immediate neighbours	ywlD, ywlB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

This gene is a member of the following regulons

DnaA regulon

The gene

Basic information

• Locus tag: BSU36950

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • conversion of L-threonine, bicarbonate/CO2 and ATP to give the intermediate L-threonylcarbamoyl-AMP (TC-AMP) and pyrophosphate as products PubMed
  • biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A), a universal modification found at position 37 of ANN decoding tRNAs, which imparts a unique structure to the anticodon loop enhancing its binding to ribosomes in vitro (shown for the corresponding E. coli protein YrdC) PubMed

• Protein family: SUA5 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1HRU (the homolog from E. coli) PubMed

• UniProt: P39153

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: tsaC PubMed

• Expression browser: tsaC PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • DnaA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: