NagB

• Description: glucosamine-6-phosphate deaminase
  
  

Gene name	nagB
Synonyms
Essential	no
Product	glucosamine-6-phosphate deaminase
Function	N-acetylglucosamine utilization
Gene expression levels in SubtiExpress: nagB
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism, Murein recycling
MW, pI	26 kDa, 5.717
Gene length, protein length	726 bp, 242 aa
Immediate neighbours	nagA, nagR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover, utilization of specific carbon sources

This gene is a member of the following regulons

NagR regulon

The gene

Basic information

• Locus tag: BSU35020

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-glucosamine 6-phosphate + H₂O = D-fructose 6-phosphate + NH₃ (according to Swiss-Prot)

• Protein family: NagB subfamily (according to Swiss-Prot)

• Paralogous protein(s): GamA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2BKXPubMed

• UniProt: O35000

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: nagA-nagB-nagR PubMed

• Expression browser: nagB PubMed

• Sigma factor:

• Regulation:
  • induced in the presence of N-acetylglucosamine (NagR) PubMed

• Regulatory mechanism:
  • NagR: transcription repression PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

Florence Vincent, Gideon J Davies, James A Brannigan
Structure and kinetics of a monomeric glucosamine 6-phosphate deaminase: missing link of the NagB superfamily?
J Biol Chem: 2005, 280(20);19649-55
[PubMed:15755726] [WorldCat.org] [DOI] (P p)

H L Mobley, R J Doyle, U N Streips, S O Langemeier
Transport and incorporation of N-acetyl-D-glucosamine in Bacillus subtilis.
J Bacteriol: 1982, 150(1);8-15
[PubMed:6174502] [WorldCat.org] [DOI] (P p)