MaeN

• Description: Na+/malate symporter
  
  

Gene name	maeN
Synonyms	yufR
Essential	no
Product	Na+/malate symporter
Function	malate uptake
Gene expression levels in SubtiExpress: maeN
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	47 kDa, 8.411
Gene length, protein length	1344 bp, 448 aa
Immediate neighbours	nupQ, yufS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

MalR regulon

The gene

Basic information

• Locus tag: BSU31580

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: sodium:citrate (SCF) symporter family (according to Swiss-Prot) 2-hydroxycarboxylate transporter family

• Paralogous protein(s): CimH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O05256

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: maeN PubMed

• Expression browser: maeN PubMed

• Sigma factor:

• Regulation: induced by malate (MalR)

• Regulatory mechanism: MalR: transcription activation

• Additional information:
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

• Mutant:
  • 1A642 (maeN::Tn917, erm) (available from the BGSC or in Jörg Stülke's lab)
  • 1A642 ( maeN::erm), PubMed, available at BGSC
  • GP1449 (erm), available in Stülke lab

• Expression vector:
  • expression of native maeN in B. subtilis: pGP1951 (in pBQ200), available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Iwona Sobczak, Juke S Lolkema
The 2-hydroxycarboxylate transporter family: physiology, structure, and mechanism.
Microbiol Mol Biol Rev: 2005, 69(4);665-95
[PubMed:16339740] [WorldCat.org] [DOI] (P p)

Original publications

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947