CwlC

• Description: N-acetylmuramoyl-L-alanine amidase
  
  

Gene name	cwlC
Synonyms
Essential	no
Product	N-acetylmuramoyl-L-alanine amidase
Function	mother cell lysis
Gene expression levels in SubtiExpress: cwlC
MW, pI	27 kDa, 5.939
Gene length, protein length	765 bp, 255 aa
Immediate neighbours	ymaB, spoVK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall/ other, sporulation proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

• Locus tag: BSU17410

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)

• Protein family: N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: contains an amidase_3 domain (like CwlD, LytC, YqiI, YrvJ)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (according to Swiss-Prot)

Database entries

• Structure: 1X60 (peptidoglycan binding domain)

• UniProt: Q06320

• KEGG entry: [3]

• E.C. number: 3.5.1.28

Expression and regulation

• Operon: cwlC PubMed

• Expression browser: cwlC PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Masaki Mishima, Toshio Shida, Kazuto Yabuki, Ken-Ichi Kato, Junichi Sekiguchi, Chojiro Kojima
Solution structure of the peptidoglycan binding domain of Bacillus subtilis cell wall lytic enzyme CwlC: characterization of the sporulation-related repeats by NMR.
Biochemistry: 2005, 44(30);10153-63
[PubMed:16042392] [WorldCat.org] [DOI] (P p)

T Shida, H Hattori, F Ise, J Sekiguchi
Mutational analysis of catalytic sites of the cell wall lytic N-acetylmuramoyl-L-alanine amidases CwlC and CwlV.
J Biol Chem: 2001, 276(30);28140-6
[PubMed:11375403] [WorldCat.org] [DOI] (P p)

T Shida, H Hattori, F Ise, J Sekiguchi
Overexpression, purification, and characterization of Bacillus subtilis N-acetylmuramoyl-L-alanine amidase CwlC.
Biosci Biotechnol Biochem: 2000, 64(7);1522-5
[PubMed:10945275] [WorldCat.org] [DOI] (P p)

A Kuroda, Y Asami, J Sekiguchi
Molecular cloning of a sporulation-specific cell wall hydrolase gene of Bacillus subtilis.
J Bacteriol: 1993, 175(19);6260-8
[PubMed:8407798] [WorldCat.org] [DOI] (P p)