LcfB

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  • Description: long-chain fatty-acid-CoA ligase, involved in surfactin production

Gene name lcfB
Synonyms yhfL
Essential no
Product long-chain fatty-acid-CoA ligase
Function fatty acid degradation
Gene expression levels in SubtiExpress: lcfB
Metabolic function and regulation of this protein in SubtiPathways:
Fatty acid degradation
MW, pI 56 kDa, 5.301
Gene length, protein length 1539 bp, 513 aa
Immediate neighbours yhfK, yhfM
Sequences Protein DNA DNA_with_flanks
Genetic context
YhfL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LcfB expression.png




























Categories containing this gene/protein

utilization of lipids

This gene is a member of the following regulons

CcpA regulon, FadR regulon

The gene

Basic information

  • Locus tag: BSU10270

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis PubMed
  • Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed