YacO

• Description: putative 23S rRNA methyltransferase
  
  

Gene name	yacO
Synonyms
Essential	no
Product	putative 23S rRNA methyltransferase
Function	unknown
Gene expression levels in SubtiExpress: yacO
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	27 kDa, 8.633
Gene length, protein length	747 bp, 249 aa
Immediate neighbours	mrnC, yacP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU00960

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: RNA methyltransferase trmH family (according to Swiss-Prot)

• Paralogous protein(s): YsgA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: Q06753

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: gltX-cysE-cysS-mrnC-yacO-yacP PubMed

• Expression browser: yacO PubMed

• Sigma factor: SigA PubMed

• Regulation: repression by cysteine (T-box) PubMed

• Regulatory mechanism: T-box, overlaps a transcription terminator upstream of cysE: transcription antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)