CysE

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  • Description: serine O-acetyltransferase

Gene name cysE
Synonyms cysA
Essential no
Product serine O-acetyltransferase
Function biosynthesis of cysteine
Gene expression levels in SubtiExpress: cysE
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging, Cys, Met & Sulfate assimilation
MW, pI 23 kDa, 9.489
Gene length, protein length 651 bp, 217 aa
Immediate neighbours gltX, cysS
Sequences Protein DNA DNA_with_flanks
Genetic context
CysE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CysE expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU00930

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine (according to Swiss-Prot)
  • Protein family: transferase hexapeptide repeat family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1T3D (from Escherichia coli, 48% identity, 64% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism: T-box, overlaps a transcription terminator upstream of cysE: transcription antitermination PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Valerie E Pye, Andrew P Tingey, Robert L Robson, Peter C E Moody
The structure and mechanism of serine acetyltransferase from Escherichia coli.
J Biol Chem: 2004, 279(39);40729-36
[PubMed:15231846] [WorldCat.org] [DOI] (P p)

Y Gagnon, R Breton, H Putzer, M Pelchat, M Grunberg-Manago, J Lapointe
Clustering and co-transcription of the Bacillus subtilis genes encoding the aminoacyl-tRNA synthetases specific for glutamate and for cysteine and the first enzyme for cysteine biosynthesis.
J Biol Chem: 1994, 269(10);7473-82
[PubMed:7510287] [WorldCat.org] (P p)