HolA

• Description: DNA polymerase III, delta subunit, part of the clamp-loader complex and the replisome
  
  

Gene name	holA
Synonyms	yqeN
Essential	yes PubMed
Product	DNA polymerase III, delta subunit
Function	DNA replication
Gene expression levels in SubtiExpress: holA
Interactions involving this protein in SubtInteract: HolA
MW, pI	40 kDa, 6.231
Gene length, protein length	1041 bp, 347 aa
Immediate neighbours	rpsT, yqzM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, essential genes

The gene

Basic information

• Locus tag: BSU25560

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • required for bacteriophage SPP1 replication PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • domain I: aa 1 - 143, RecA-like fold PubMed
  • domain II: aa 144 - 214, four alpha helices PubMed
  • domain III: aa 215 - 339, seven alpha helices PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:#
  • DnaX(3)-HolA-HolB, the clamp-loader complex PubMed
  • part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
  • DnaE-HolA PubMed

• Localization: Nucleoid (Mid-cell) PubMed

Database entries

• Structure: 3ZH9 PubMed

• UniProt: P54459

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: holA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: