ParB

• Description: chromosome positioning near the pole and transport through the polar septum / antagonist of Soj-dependent inhibition of sporulation initiation
  
  

Gene name	spo0J
Synonyms	spo0JB
Essential	no
Product	site-specific DNA-binding protein
Function	chromosome positioning before asymmetric septation, specifying its orientation, and imposing directionality on its subsequent transport through the septum; required for sporulation (antagonist of Soj-dependent inhibition of sporulation initiation)
Gene expression levels in SubtiExpress: spo0J specifying its orientation, and imposing directionality on its subsequent transport through the septum; required for sporulation (antagonist of Soj-dependent inhibition of sporulation initiation)
Interactions involving this protein in SubtInteract: Spo0J
MW, pI	32 kDa, 8.417
Gene length, protein length	846 bp, 282 aa
Immediate neighbours	yyaC, soj
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA condensation/ segregation, sporulation/ other

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

• Locus tag: BSU40960

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: inhibits Soj dimerization and concomitant DNA-binding activity by stimulating Soj ATPase activity PubMed

• Protein family: parB family (according to Swiss-Prot)

• Paralogous protein(s): Noc

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: Spo0J-Soj PubMed

• Localization:
  • chromosome centromer PubMed

Database entries

• Structure:

• UniProt: P26497

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: soj-spo0J (according to DBTBS)

• Expression browser: spo0J PubMed

• Sigma factor:

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1S135 ( spo0J::spec), PubMed, available at BGSC
  • 1S136 ( spo0J::spec), PubMed, available at BGSC
  • 1S137 ( spo0J::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed