YngG

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  • Description: 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) lyase

Gene name yngG
Synonyms
Essential no
Product 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) lyase
Function mother cell metabolism, leucine utilization
Gene expression levels in SubtiExpress: yngG
MW, pI 32 kDa, 5.753
Gene length, protein length 897 bp, 299 aa
Immediate neighbours yngF, yngHB
Sequences Protein DNA Advanced_DNA
Genetic context
YngG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YngG expression.png















Categories containing this gene/protein

utilization of amino acids, sporulation proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

  • Locus tag: BSU18230

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-3-hydroxy-3-methylglutaryl-CoA = acetyl-CoA + acetoacetate PubMed
  • Protein family: HMG-CoA lyase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed during sporulation in the mother cell (SigE) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Tzu-Lin Hsiao, Olga Revelles, Lifeng Chen, Uwe Sauer, Dennis Vitkup
Automatic policing of biochemical annotations using genomic correlations.
Nat Chem Biol: 2010, 6(1);34-40
[PubMed:19935659] [WorldCat.org] [DOI] (I p)

Raghavendra Joshi, Brian B McSpadden Gardener
Identification and Characterization of Novel Genetic Markers Associated with Biological Control Activities in Bacillus subtilis.
Phytopathology: 2006, 96(2);145-54
[PubMed:18943917] [WorldCat.org] [DOI] (P p)

Farhad Forouhar, Munif Hussain, Ramy Farid, Jordi Benach, Mariam Abashidze, William C Edstrom, Sergey M Vorobiev, Rong Xiao, Thomas B Acton, Zhuji Fu, Jung-Ja P Kim, Henry M Miziorko, Gaetano T Montelione, John F Hunt
Crystal structures of two bacterial 3-hydroxy-3-methylglutaryl-CoA lyases suggest a common catalytic mechanism among a family of TIM barrel metalloenzymes cleaving carbon-carbon bonds.
J Biol Chem: 2006, 281(11);7533-45
[PubMed:16330546] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)