ValS

• Description: valyl-tRNA synthetase
  
  

Gene name	valS
Synonyms
Essential	yes PubMed
Product	valyl-tRNA synthetase
Function	translation
Gene expression levels in SubtiExpress: valS
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging, Folate
MW, pI	101 kDa, 4.868
Gene length, protein length	2640 bp, 880 aa
Immediate neighbours	folC, yszA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box, Efp-dependent proteins

The gene

Basic information

• Locus tag: BSU28090

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val) (according to Swiss-Prot)

• Protein family: ValS type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1IYW (from Thermus thermophilus, 41% identity, 60% similarity) PubMed

• UniProt: Q05873

• KEGG entry: [3]

• E.C. number: 6.1.1.9

Expression and regulation

• Operon: valS-folC PubMed

• Expression browser: valS PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by valine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: riboswitch, transcription termination/antitermination PubMed

• Additional information:
  • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Original Publications

D Luo, J Leautey, M Grunberg-Manago, H Putzer
Structure and regulation of expression of the Bacillus subtilis valyl-tRNA synthetase gene.
J Bacteriol: 1997, 179(8);2472-8
[PubMed:9098041] [WorldCat.org] [DOI] (P p)