YlaM

• Description: glutaminase, high affinity for glutamine
  
  

Gene name	ylaM
Synonyms
Essential	no
Product	glutaminase
Function	glutamine degradation
Gene expression levels in SubtiExpress: ylaM
MW, pI	33 kDa, 5.676
Gene length, protein length	927 bp, 309 aa
Immediate neighbours	ylaL, ylaN
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids, sporulation proteins

The gene

Basic information

• Locus tag: BSU14830

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-glutamine + H₂O = L-glutamate + NH₃ (according to Swiss-Prot)

• Protein family: glutaminase family (according to Swiss-Prot)

• Paralogous protein(s): GlsA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3BRM (the structure of GlsA) PubMed

• UniProt: O07637

• KEGG entry: [2]

• E.C. number: 3.5.1.2

Expression and regulation

• Operon:

• Expression browser: ylaM PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Greg Brown, Alex Singer, Michael Proudfoot, Tatiana Skarina, Youngchang Kim, Changsoo Chang, Irina Dementieva, Ekaterina Kuznetsova, Claudio F Gonzalez, Andrzej Joachimiak, Alexei Savchenko, Alexander F Yakunin
Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis.
Biochemistry: 2008, 47(21);5724-35
[PubMed:18459799] [WorldCat.org] [DOI] (I p)