McpC

• Description: membrane-bound chemotaxis receptor for proline, methyl-accepting chemotaxis protein
  
  

Gene name	mcpC
Synonyms	prg71
Essential	no
Product	methyl-accepting chemotaxis protein
Function	control of chemotaxis threonine, glycine, serine, lysine, valine and arginine
Gene expression levels in SubtiExpress: mcpC
Interactions involving this protein in SubtInteract: McpC
MW, pI	71 kDa, 5.174
Gene length, protein length	1962 bp, 654 aa
Immediate neighbours	ykwB, ykwC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

• Locus tag: BSU13950

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • deaminated McpC stimulates phosphorylation of CheA PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • deamination of Gln-609 (by CheD) PubMed, deaminated McpC stimulates phosphorylation of CheA PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • CheD-McpC, deamination of Gln-609 in McpC PubMed
  • McpC-ArtP, for chemotaxis to lysine and arginine PubMed
  • McpC-MetQ, for chemotaxis to methionine PubMed
  • McpC-YckB, for chemotaxis to tryptophan PubMed

• Localization:
  • cell membrane PubMed

Database entries

• Structure:

• UniProt: P54576

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mcpC PubMed

• Expression browser: mcpC PubMed

• Sigma factor: SigD PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • in minimal medium, McpC is present with 2,800 +/- 640 molecules per cell PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Vincent J Cannistraro, George D Glekas, Christopher V Rao, George W Ordal
Cellular stoichiometry of the chemotaxis proteins in Bacillus subtilis.
J Bacteriol: 2011, 193(13);3220-7
[PubMed:21515776] [WorldCat.org] [DOI] (I p)

George D Glekas, Matthew J Plutz, Hanna E Walukiewicz, George M Allen, Christopher V Rao, George W Ordal
Elucidation of the multiple roles of CheD in Bacillus subtilis chemotaxis.
Mol Microbiol: 2012, 86(3);743-56
[PubMed:22931217] [WorldCat.org] [DOI] (I p)