XylB

• Description: xylulokinase
  
  

Gene name	xylB
Synonyms	yncA
Essential	no
Product	xylulokinase
Function	utilization of xylan and xylose
Gene expression levels in SubtiExpress: xylB
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	55 kDa, 6.459
Gene length, protein length	1497 bp, 499 aa
Immediate neighbours	xylA, yncB
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, XylR regulon

The gene

Basic information

• Locus tag: BSU17610

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + D-xylulose = ADP + D-xylulose 5-phosphate (according to Swiss-Prot)

• Protein family: FGGY kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2NLX (from E. coli, 35% identity, 52% similarity) PubMed

• UniProt: P39211

• KEGG entry: [3]

• E.C. number: 2.7.1.17

Expression and regulation

• Operon: xylA-xylB PubMed

• Expression browser: xylB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • carbon catabolite repression (CcpA) PubMed
  • induction by xylose (XylR) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • XylR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP1151 (del aphA3) available in Stülke lab
  • 1A719 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Wolfgang Hillen, Erlangen University, Germany Homepage

References

Eric Di Luccio, Barbara Petschacher, Jennifer Voegtli, Hui-Ting Chou, Henning Stahlberg, Bernd Nidetzky, David K Wilson
Structural and kinetic studies of induced fit in xylulose kinase from Escherichia coli.
J Mol Biol: 2007, 365(3);783-98
[PubMed:17123542] [WorldCat.org] [DOI] (P p)

A Kraus, C Hueck, D Gärtner, W Hillen
Catabolite repression of the Bacillus subtilis xyl operon involves a cis element functional in the context of an unrelated sequence, and glucose exerts additional xylR-dependent repression.
J Bacteriol: 1994, 176(6);1738-45
[PubMed:8132469] [WorldCat.org] [DOI] (P p)

S Jacob, R Allmansberger, D Gärtner, W Hillen
Catabolite repression of the operon for xylose utilization from Bacillus subtilis W23 is mediated at the level of transcription and depends on a cis site in the xylA reading frame.
Mol Gen Genet: 1991, 229(2);189-96
[PubMed:1921970] [WorldCat.org] [DOI] (P p)

D Gärtner, M Geissendörfer, W Hillen
Expression of the Bacillus subtilis xyl operon is repressed at the level of transcription and is induced by xylose.
J Bacteriol: 1988, 170(7);3102-9
[PubMed:2454911] [WorldCat.org] [DOI] (P p)