YxeM

• Description: putative cysteine ABC transporter (binding protein)
  
  

Gene name	yxeM
Synonyms
Essential	no
Product	putative cysteine ABC transporter (binding protein)
Function	uptake of cysteine
Gene expression levels in SubtiExpress: yxeM
Interactions involving this protein in SubtInteract: YxeM
MW, pI	29 kDa, 9.086
Gene length, protein length	792 bp, 264 aa
Immediate neighbours	yxeN, yxeL
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, biosynthesis/ acquisition of amino acids, membrane proteins

This gene is a member of the following regulons

CymR regulon

The gene

Basic information

• Locus tag: BSU39500

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: bacterial solute-binding protein 3 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • YxeO-YxeN-YxeM PubMed

• Localization: associated to the membrane (via YxeN) PubMed

Database entries

• Structure:

• UniProt: P54952

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yxeK-yxeL-yxeM-yxeN-yxeO-yxeP-yxeQ PubMed

• Expression browser: yxeM PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of cysteine (CymR) PubMed

• Regulatory mechanism:
  • CymR: transcription repression PubMed

• Additional information:
  • the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed

Biological materials

• Mutant:
  • 1A950 ( yxeM::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947