PatB

• Description: cystathione-beta-lyase
  
  

Gene name	patB
Synonyms
Essential	no
Product	cystathione-beta-lyase
Function	biosynthesis of methionine
Gene expression levels in SubtiExpress: patB
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	42 kDa, 5.439
Gene length, protein length	1161 bp, 387 aa
Immediate neighbours	yugE, kinB
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The gene

Basic information

• Locus tag: BSU31440

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-cystathionine + H₂O = L-homocysteine + NH₃ + pyruvate (according to Swiss-Prot)

• Protein family: MalY/patB cystathionine beta-lyase subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3T32 (from B. anthracis, 46% identity, 75% similarity)

• UniProt: Q08432

• KEGG entry: [3]

• E.C. number: 4.4.1.8

Expression and regulation

• Operon: patB PubMed

• Expression browser: patB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A952 ( patB::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

S Auger, M P Gomez, A Danchin, I Martin-Verstraete
The PatB protein of Bacillus subtilis is a C-S-lyase.
Biochimie: 2005, 87(2);231-8
[PubMed:15760717] [WorldCat.org] [DOI] (P p)

Bauke Oudega, Gregory Koningstein, Luísa Rodrigues, Maria de Sales Ramon, Helmut Hilbert, Andreas Düsterhöft, Thomas M Pohl, Thomas Weitzenegger
Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai).
Microbiology (Reading): 1997, 143 ( Pt 8);2769-2774
[PubMed:9274030] [WorldCat.org] [DOI] (P p)