SpsI

From SubtiWiki
Revision as of 16:03, 18 September 2012 by Jstuelk (talk | contribs)
Jump to: navigation, search
  • Description: glucose-1-phosphate thymidylyltransferase, spore coat polysaccharide synthesis

Gene name spsI
Synonyms ipa-71d
Essential no
Product glucose-1-phosphate thymidylyltransferase
Function rhamnose biosynthesis, spore coat polysaccharide synthesis
Gene expression levels in SubtiExpress: spsI
MW, pI 27 kDa, 5.385
Gene length, protein length 738 bp, 246 aa
Immediate neighbours spsJ, spsG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SpsI context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SpsI expression.png















Categories containing this gene/protein

miscellaneous metabolic pathways, sporulation proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

  • Locus tag: BSU37840

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • alpha-D-glucose-1-phosphate + dTTP --> dTDP-glucose PubMed
  • Protein family: glucose-1-phosphate thymidylyltransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed late during sporulation in the mother cell (SigK) PubMed
  • Regulatory mechanism:
  • Additional information:
    • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References