PMAD

pMAD: click to enlarge

General comments

Integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping the control of the expression under its natural promotor

• E.coli: multicopy plasmid, select for ampicillin
• B. subtilis: no ori, select for spectinomycin

Hints for the usage

• for a markerless deletion of a gene amplify 1000 bp up- and downstream of the gene and clone them into the MCS of the plasmid

The reference