GanB

• Description: endo-1,4-beta-galactosidase
  
  

Gene name	ganB
Synonyms	yvfO
Essential	no
Product	endo-1,4-beta-galactosidase
Function	galactan utilization
Gene expression levels in SubtiExpress: ganB
MW, pI	47 kDa, 8.243
Gene length, protein length	1287 bp, 429 aa
Immediate neighbours	rsbP, ganA
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

LacR regulon

The gene

Basic information

• Locus tag: BSU34120

Phenotypes of a mutant

• a cycB-ganP-ganQ-ganA-ganB mutant is impaired in the utilization of galactan PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endohydrolysis of (1->4)-beta-D-galactosidic linkages in galactan, generation of galactotriose PubMed

• Protein family: glycosyl hydrolase 53 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • likely to be extrecellular PubMed

Database entries

• Structure: 2J74 (B. licheniformis)

• UniProt: O07013

• KEGG entry: [3]

• E.C. number: 3.2.1.89

Expression and regulation

• Operon: cycB-ganP-ganQ-ganA-ganB PubMed

• Expression browser: ganB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • LacR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yunrong Chai, Pascale B Beauregard, Hera Vlamakis, Richard Losick, Roberto Kolter
Galactose metabolism plays a crucial role in biofilm formation by Bacillus subtilis.
mBio: 2012, 3(4);e00184-12
[PubMed:22893383] [WorldCat.org] [DOI] (I e)

Stephanie Shipkowski, Jean E Brenchley
Bioinformatic, genetic, and biochemical evidence that some glycoside hydrolase family 42 beta-galactosidases are arabinogalactan type I oligomer hydrolases.
Appl Environ Microbiol: 2006, 72(12);7730-8
[PubMed:17056685] [WorldCat.org] [DOI] (P p)

R A Daniel, J Haiech, F Denizot, J Errington
Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR.
J Bacteriol: 1997, 179(17);5636-8
[PubMed:9287030] [WorldCat.org] [DOI] (P p)