CycB

• Description: galactotriose ABC transporter, binding protein
  
  

Gene name	cycB
Synonyms	yvfK
Essential	no
Product	galactotriose ABC transporter, binding protein
Function	uptake of galactotriose
Gene expression levels in SubtiExpress: cycB
Interactions involving this protein in SubtInteract: CycB
MW, pI	45 kDa, 5.441
Gene length, protein length	1263 bp, 421 aa
Immediate neighbours	ganP, lacR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

LacR regulon

The gene

Basic information

• Locus tag: BSU34160

Phenotypes of a mutant

• a cycB-ganP-ganQ-ganA-ganB mutant is impaired in the utilization of galactan PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: bacterial solute-binding protein 1 family (according to Swiss-Prot)

• Paralogous protein(s): MdxE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • (GanP-GanQ)-CycB PubMed

• Localization: cell membrane (via YvfL-YvfM) PubMed

Database entries

• Structure:

• UniProt: O07009

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: cycB-ganP-ganQ-ganA-ganB PubMed

• Expression browser: cycB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • LacR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yunrong Chai, Pascale B Beauregard, Hera Vlamakis, Richard Losick, Roberto Kolter
Galactose metabolism plays a crucial role in biofilm formation by Bacillus subtilis.
mBio: 2012, 3(4);e00184-12
[PubMed:22893383] [WorldCat.org] [DOI] (I e)

A Kamionka, M K Dahl
Bacillus subtilis contains a cyclodextrin-binding protein which is part of a putative ABC-transporter.
FEMS Microbiol Lett: 2001, 204(1);55-60
[PubMed:11682178] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

R A Daniel, J Haiech, F Denizot, J Errington
Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR.
J Bacteriol: 1997, 179(17);5636-8
[PubMed:9287030] [WorldCat.org] [DOI] (P p)