Spo0E

• Description: Spo0A-P phosphatase, control of the phosphorelay
  
  

Gene name	spo0E
Synonyms
Essential	no
Product	Spo0A-P phosphatase
Function	initiation of sporulation
Gene expression levels in SubtiExpress: spo0E
Interactions involving this protein in SubtInteract: Spo0E
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	9 kDa, 6.483
Gene length, protein length	255 bp, 85 aa
Immediate neighbours	ykvA, eag
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, phosphorelay

This gene is a member of the following regulons

SigB regulon, AbrB regulon, Rok regulon

The gene

Basic information

• Locus tag: BSU13640

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: spo0E family (according to Swiss-Prot)

• Paralogous protein(s): YnzD

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • Spo0F-Spo0E PubMed
  • Spo0A-Spo0E PubMed
  • FtsH-Spo0E PubMed

• Localization:

Database entries

• Structure:

• UniProt: P05043

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spo0E PubMed

• Expression browser: spo0E PubMed

• Sigma factor:
  • SigA PubMed, SigB PubMed

• Regulation:
  • repressed during logrithmic growth (AbrB) PubMed
  • induced under stress conditions (SigB) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed
  • Rok: transcription repression of the SigB-dependent promoter PubMed

• Additional information:
  • Spo0E is degraded by FtsH
  • stress-induced expression of Spo0E by SigB results in prevention of sporulation during stress (due to dephosphorylation of Spo0A) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Tony Wilkinson, York University, U.K. homepage

References

Reviews

Daniel Schultz, Peter G Wolynes, Eshel Ben Jacob, José N Onuchic
Deciding fate in adverse times: sporulation and competence in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2009, 106(50);21027-34
[PubMed:19995980] [WorldCat.org] [DOI] (I p)

Original Publications

Additional publications: PubMed

Ai Thi Thuy Le, Wolfgang Schumann
The Spo0E phosphatase of Bacillus subtilis is a substrate of the FtsH metalloprotease.
Microbiology (Reading): 2009, 155(Pt 4);1122-1132
[PubMed:19332814] [WorldCat.org] [DOI] (P p)

Shigeo Hosoya, Kei Asai, Naotake Ogasawara, Michio Takeuchi, Tsutomu Sato
Mutation in yaaT leads to significant inhibition of phosphorelay during sporulation in Bacillus subtilis.
J Bacteriol: 2002, 184(20);5545-53
[PubMed:12270811] [WorldCat.org] [DOI] (P p)

Andrea Feucht, Laura Abbotts, Jeffery Errington
The cell differentiation protein SpoIIE contains a regulatory site that controls its phosphatase activity in response to asymmetric septation.
Mol Microbiol: 2002, 45(4);1119-30
[PubMed:12180929] [WorldCat.org] [DOI] (P p)

Sophie J Stephenson, Marta Perego
Interaction surface of the Spo0A response regulator with the Spo0E phosphatase.
Mol Microbiol: 2002, 44(6);1455-67
[PubMed:12067336] [WorldCat.org] [DOI] (P p)

H Nanamiya, K Takahashi, M Fujita, F Kawamura
Deficiency of the initiation events of sporulation in Bacillus subtilis clpP mutant can be suppressed by a lack of the Spo0E protein phosphatase.
Biochem Biophys Res Commun: 2000, 279(1);229-33
[PubMed:11112444] [WorldCat.org] [DOI] (P p)

I Lucet, A Feucht, M D Yudkin, J Errington
Direct interaction between the cell division protein FtsZ and the cell differentiation protein SpoIIE.
EMBO J: 2000, 19(7);1467-75
[PubMed:10747015] [WorldCat.org] [DOI] (P p)

K L Ohlsen, J K Grimsley, J A Hoch
Deactivation of the sporulation transcription factor Spo0A by the Spo0E protein phosphatase.
Proc Natl Acad Sci U S A: 1994, 91(5);1756-60
[PubMed:8127878] [WorldCat.org] [DOI] (P p)

M Perego, J A Hoch
Negative regulation of Bacillus subtilis sporulation by the spo0E gene product.
J Bacteriol: 1991, 173(8);2514-20
[PubMed:1901567] [WorldCat.org] [DOI] (P p)

U Bai, M Lewandoski, E Dubnau, I Smith
Temporal regulation of the Bacillus subtilis early sporulation gene spo0F.
J Bacteriol: 1990, 172(9);5432-9
[PubMed:2118512] [WorldCat.org] [DOI] (P p)

M A Strauch, G B Spiegelman, M Perego, W C Johnson, D Burbulys, J A Hoch
The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein.
EMBO J: 1989, 8(5);1615-21
[PubMed:2504584] [WorldCat.org] [DOI] (P p)

M Perego, J A Hoch
Isolation and sequence of the spo0E gene: its role in initiation of sporulation in Bacillus subtilis.
Mol Microbiol: 1987, 1(1);125-32
[PubMed:2838724] [WorldCat.org] [DOI] (P p)