MprF

• Description: synthesis of lysylphosphatidylglycerol (L-PG), lysinylation of phosphatidylglycerol provides some resistance against positively charged antimicrobial peptides
  
  

Gene name	mprF
Synonyms	yfiW, yfiX
Essential	no
Product	lysyl-phosphatidylglycerol synthase
Function	lysyl-phosphatidylglycerol lipid biosynthesis
Gene expression levels in SubtiExpress: mprF
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis
MW, pI	96 kDa, 9.498
Gene length, protein length	2568 bp, 856 aa
Immediate neighbours	yfiV, yfiY
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids, membrane proteins

The gene

Basic information

• Locus tag: BSU08425

Database entries

• DBTBS entry: [1]

• SubtiList entry: old_YfiWold_YfiX

Additional information

Gene was re-assembled due to sequencing error in original sequence (it encompasses the genes annotated as yfiX and yfiW in SubtiList) PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane at the septum PubMed

Database entries

• Structure:

• UniProt: C0H3X7

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: mprF (according to DBTBS)

• Expression browser: mprF PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hervé Roy, Michael Ibba
Broad range amino acid specificity of RNA-dependent lipid remodeling by multiple peptide resistance factors.
J Biol Chem: 2009, 284(43);29677-83
[PubMed:19734140] [WorldCat.org] [DOI] (I p)

Anna-Barbara Hachmann, Esther R Angert, John D Helmann
Genetic analysis of factors affecting susceptibility of Bacillus subtilis to daptomycin.
Antimicrob Agents Chemother: 2009, 53(4);1598-609
[PubMed:19164152] [WorldCat.org] [DOI] (I p)

Letal I Salzberg, John D Helmann
Phenotypic and transcriptomic characterization of Bacillus subtilis mutants with grossly altered membrane composition.
J Bacteriol: 2008, 190(23);7797-807
[PubMed:18820022] [WorldCat.org] [DOI] (I p)

Hervé Roy, Michael Ibba
RNA-dependent lipid remodeling by bacterial multiple peptide resistance factors.
Proc Natl Acad Sci U S A: 2008, 105(12);4667-72
[PubMed:18305156] [WorldCat.org] [DOI] (I p)

Ayako Nishibori, Jin Kusaka, Hiroshi Hara, Masato Umeda, Kouji Matsumoto
Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes.
J Bacteriol: 2005, 187(6);2163-74
[PubMed:15743965] [WorldCat.org] [DOI] (P p)

Petra Staubitz, Andreas Peschel
MprF-mediated lysinylation of phospholipids in Bacillus subtilis--protection against bacteriocins in terrestrial habitats?
Microbiology (Reading): 2002, 148(Pt 11);3331-3332
[PubMed:12427923] [WorldCat.org] [DOI] (P p)