YesW

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  • Description: rhamnogalacturonan lyase, generates oligosaccharides

Gene name yesW
Synonyms
Essential no
Product rhamnogalacturonan lyase, generates oligosaccharides
Function utilization of rhamnogalacturonan
Gene expression levels in SubtiExpress: yesW
MW, pI 67 kDa, 5.966
Gene length, protein length 1860 bp, 620 aa
Immediate neighbours yesV, yesX
Sequences Protein DNA Advanced_DNA
Genetic context
YesW context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YesW expression.png
























Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

YesS regulon

The gene

Basic information

  • Locus tag: BSU07050

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: FG-GAP repeat (according to Swiss-Prot)
  • Paralogous protein(s): YesX

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulatory mechanism: YesS: transcriptional activation PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Sandrine Poncet, Maryline Soret, Peggy Mervelet, Josef Deutscher, Philippe Noirot
Transcriptional activator YesS is stimulated by histidine-phosphorylated HPr of the Bacillus subtilis phosphotransferase system.
J Biol Chem: 2009, 284(41);28188-28197
[PubMed:19651770] [WorldCat.org] [DOI] (I p)

Akihito Ochiai, Takafumi Itoh, Bunzo Mikami, Wataru Hashimoto, Kousaku Murata
Structural determinants responsible for substrate recognition and mode of action in family 11 polysaccharide lyases.
J Biol Chem: 2009, 284(15);10181-9
[PubMed:19193638] [WorldCat.org] [DOI] (P p)

Akihito Ochiai, Takafumi Itoh, Yukie Maruyama, Akiko Kawamata, Bunzo Mikami, Wataru Hashimoto, Kousaku Murata
A novel structural fold in polysaccharide lyases: Bacillus subtilis family 11 rhamnogalacturonan lyase YesW with an eight-bladed beta-propeller.
J Biol Chem: 2007, 282(51);37134-45
[PubMed:17947240] [WorldCat.org] [DOI] (P p)

Akihito Ochiai, Takafumi Itoh, Akiko Kawamata, Wataru Hashimoto, Kousaku Murata
Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization.
Appl Environ Microbiol: 2007, 73(12);3803-13
[PubMed:17449691] [WorldCat.org] [DOI] (P p)

Akihito Ochiai, Masayuki Yamasaki, Takafumi Itoh, Bunzo Mikami, Wataru Hashimoto, Kousaku Murata
Crystallization and preliminary X-ray analysis of the rhamnogalacturonan lyase YesW from Bacillus subtilis strain 168, a member of polysaccharide lyase family 11.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2006, 62(Pt 5);438-40
[PubMed:16682770] [WorldCat.org] [DOI] (I p)