FabF

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  • Description: beta-ketoacyl-acyl carrier protein synthase II, involved in the control of membrane fluidity

Gene name fabF
Synonyms yjaY
Essential yes PubMed
Product beta-ketoacyl-acyl carrier protein synthase II
Function fatty acid biosynthesis
Gene expression levels in SubtiExpress: fabF
Metabolic function and regulation of this protein in SubtiPathways:
Lipid synthesis
MW, pI 43 kDa, 4.768
Gene length, protein length 1239 bp, 413 aa
Immediate neighbours fabHA, yjaZ
Sequences Protein DNA Advanced_DNA
Genetic context
FabF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FabF expression.png




























Categories containing this gene/protein

membrane dynamics, biosynthesis of lipids, cell envelope stress proteins (controlled by SigM, V, W, X, Y), essential genes

This gene is a member of the following regulons

FapR regulon, SigW regulon

The gene

Basic information

  • Locus tag: BSU11340

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • (Z)-hexadec-11-enoyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = (Z)-3-oxooctadec-13-enoyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein] (according to Swiss-Prot)
    • SigW-dependent expression of fabF and the yuaF-floT-yuaI operon result in reduced membrane fluidity PubMed
  • Protein family: beta-ketoacyl-ACP synthases family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1OX0 (the protein from Streptococcus pneumoniae) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • fabHA: expressed when the cells experience a lack of malonyl-CoA (FapR) PubMed
    • fabHA: induced upon fatty acid biosynthesis inhibition PubMed
    • fabF: induced by alkaline shock and by polymyxin B, vancomycin, cephalosporin C, D-cycloserine, and triton X-100 (SigW) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

Stephen W White, Jie Zheng, Yong-Mei Zhang, Rock
The structural biology of type II fatty acid biosynthesis.
Annu Rev Biochem: 2005, 74;791-831
[PubMed:15952903] [WorldCat.org] [DOI] (P p)

Original Publications

Additional publications: PubMed