SmpB

• Description: tmRNA-binding protein
  
  

Gene name	smpB
Synonyms	yvaI
Essential	no
Product	tmRNA-binding protein
Function	trans-translation
Gene expression levels in SubtiExpress: smpB
MW, pI	17 kDa, 10.421
Gene length, protein length	468 bp, 156 aa
Immediate neighbours	ssrA, rnr
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

The gene

Basic information

• Locus tag: BSU33600

Phenotypes of a mutant

• impaired growth at high and low temperatures PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: smpB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SmpB-SsrA

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O32230

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:
  • secG-yvaK-rnr-smpB-ssrA PubMed
  • yvaK-rnr-smpB-ssrA PubMed
  • smpB-ssrA PubMed

• Expression browser: smpB PubMed

• Sigma factor:

• Regulation:
  • yvaK: SigB PubMed

• Regulation:
  • yvaK: induced by stress (SigB) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Chet Price, Davis, USA homepage

References

Hiromi Ujiie, Tomoko Matsutani, Hisashi Tomatsu, Ai Fujihara, Chisato Ushida, Yasuhiko Miwa, Yasutaro Fujita, Hyouta Himeno, Akira Muto
Trans-translation is involved in the CcpA-dependent tagging and degradation of TreP in Bacillus subtilis.
J Biochem: 2009, 145(1);59-66
[PubMed:18977770] [WorldCat.org] [DOI] (I p)

Ji-Hyun Shin, Chester W Price
The SsrA-SmpB ribosome rescue system is important for growth of Bacillus subtilis at low and high temperatures.
J Bacteriol: 2007, 189(10);3729-37
[PubMed:17369301] [WorldCat.org] [DOI] (P p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Jeffrey H Withey, David I Friedman
A salvage pathway for protein structures: tmRNA and trans-translation.
Annu Rev Microbiol: 2003, 57;101-23
[PubMed:12730326] [WorldCat.org] [DOI] (P p)

T Wiegert, W Schumann
SsrA-mediated tagging in Bacillus subtilis.
J Bacteriol: 2001, 183(13);3885-9
[PubMed:11395451] [WorldCat.org] [DOI] (P p)

A W Karzai, E D Roche, R T Sauer
The SsrA-SmpB system for protein tagging, directed degradation and ribosome rescue.
Nat Struct Biol: 2000, 7(6);449-55
[PubMed:10881189] [WorldCat.org] [DOI] (P p)