MetP

• Description: methionine ABC transporter, permease
  
  

Gene name	metP
Synonyms	yusB
Essential	no
Product	methionine ABC transporter, permease
Function	methionine uptake
Gene expression levels in SubtiExpress: metP
Interactions involving this protein in SubtInteract: MetP
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	23 kDa, 6.618
Gene length, protein length	666 bp, 222 aa
Immediate neighbours	metQ, metN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, biosynthesis/ acquisition of amino acids, membrane proteins

This gene is a member of the following regulons

CodY regulon, S-box

The gene

Basic information

• Locus tag: BSU32740

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • MetN-MetP-MetQ PubMed

• Localization: cell membrane PubMed

Database entries

• Structure:

• UniProt: O32168

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: metN-metP-metQ PubMed

• Expression browser: metP PubMed

• Sigma factor:

• Regulation:
  • activated during growth in the presence of branched chain amino acids (CodY) PubMed
  • induced by methionine starvation (S-box) PubMed

• Regulatory mechanism:
  • S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed
  • CodY: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A797 ( metP::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jerneja Tomsic, Brooke A McDaniel, Frank J Grundy, Tina M Henkin
Natural variability in S-adenosylmethionine (SAM)-dependent riboswitches: S-box elements in bacillus subtilis exhibit differential sensitivity to SAM In vivo and in vitro.
J Bacteriol: 2008, 190(3);823-33
[PubMed:18039762] [WorldCat.org] [DOI] (I p)

Marie-Françoise Hullo, Sandrine Auger, Elie Dassa, Antoine Danchin, Isabelle Martin-Verstraete
The metNPQ operon of Bacillus subtilis encodes an ABC permease transporting methionine sulfoxide, D- and L-methionine.
Res Microbiol: 2004, 155(2);80-6
[PubMed:14990259] [WorldCat.org] [DOI] (P p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

F J Grundy, T M Henkin
The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in gram-positive bacteria.
Mol Microbiol: 1998, 30(4);737-49
[PubMed:10094622] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)