TrmB

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  • Description: tRNA (m7G46) methyltransferase

Gene name trmB
Synonyms ytmQ
Essential no
Product tRNA (m7G46) methyltransferase
Function tRNA modification
Gene expression levels in SubtiExpress: trmB
MW, pI 24 kDa, 6.351
Gene length, protein length 639 bp, 213 aa
Immediate neighbours ytnP, ytzH
Sequences Protein DNA Advanced_DNA
Genetic context
YtmQ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TrmB expression.png
























Categories containing this gene/protein

translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU29900

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine (according to Swiss-Prot)
  • Protein family: TrmB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ingrid Zegers, Daniel Gigot, Françoise van Vliet, Catherine Tricot, Stéphane Aymerich, Janusz M Bujnicki, Jan Kosinski, Louis Droogmans
Crystal structure of Bacillus subtilis TrmB, the tRNA (m7G46) methyltransferase.
Nucleic Acids Res: 2006, 34(6);1925-34
[PubMed:16600901] [WorldCat.org] [DOI] (I e)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)