IlvC

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  • Description: ketol-acid reductoisomerase (2,3-dihydroxy-3-methylbutanoate, 2-acetolactate)

Gene name ilvC
Synonyms
Essential no
Product ketol-acid reductoisomerase
(2,3-dihydroxy-3-methylbutanoate, 2-acetolactate)
Function biosynthesis of branched-chain amino acids
Gene expression levels in SubtiExpress: ilvC
Metabolic function and regulation of this protein in SubtiPathways:
Ile, Leu, Val, Coenzyme A
MW, pI 37 kDa, 5.37
Gene length, protein length 1026 bp, 342 aa
Immediate neighbours leuA, ilvH
Sequences Protein DNA Advanced_DNA
Genetic context
IlvC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
IlvC expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, CodY regulon, T-box, TnrA regulon

The gene

Basic information

  • Locus tag: BSU28290

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH (according to Swiss-Prot)
  • Protein family: ketol-acid reductoisomerase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylated on Arg-298 PubMed
    • phosphorylated on Ser-338 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1NP3 (from Escherichia coli, 53% identity, 71% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: for a complete overview on the regulation of the ilv operon, see Brinsmade et al.
    • repressed by casamino acids PubMed
    • expression is stimulated in the presence of glucose PubMed
    • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY, T-box) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion: pGP521 (in pAC5), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References