YsxC

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  • Description: assembly of the 50S subunit of the ribosome

Gene name ysxC
Synonyms orfX
Essential yes PubMed
Product GTPase
Function ribosome assembly
Gene expression levels in SubtiExpress: ysxC
Interactions involving this protein in SubtInteract: YsxC
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 21 kDa, 9.708
Gene length, protein length 585 bp, 195 aa
Immediate neighbours ysxD, lonA
Sequences Protein DNA Advanced_DNA
Genetic context
YsxC context.gif
Expression at a glance   PubMed
YsxC expression.png




























Categories containing this gene/protein

translation, essential genes, membrane proteins, GTP-binding proteins

This gene is a member of the following regulons

CtsR regulon

The gene

Basic information

  • Locus tag: BSU28190

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: engB family (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1SVI (complex with GDP), 1SUL
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP834, available in Stülke lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP842, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

Your additional remarks

References

Reviews

Natalie Verstraeten, Maarten Fauvart, Wim Versées, Jan Michiels
The universally conserved prokaryotic GTPases.
Microbiol Mol Biol Rev: 2011, 75(3);507-42, second and third pages of table of contents
[PubMed:21885683] [WorldCat.org] [DOI] (I p)

Robert A Britton
Role of GTPases in bacterial ribosome assembly.
Annu Rev Microbiol: 2009, 63;155-76
[PubMed:19575570] [WorldCat.org] [DOI] (I p)


Original publications

Additional publications: PubMed

Catherine Wicker-Planquart, Anne-Emmanuelle Foucher, Mathilde Louwagie, Robert A Britton, Jean-Michel Jault
Interactions of an essential Bacillus subtilis GTPase, YsxC, with ribosomes.
J Bacteriol: 2008, 190(2);681-90
[PubMed:17981968] [WorldCat.org] [DOI] (I p)

Laura Schaefer, William C Uicker, Catherine Wicker-Planquart, Anne-Emmanuelle Foucher, Jean-Michel Jault, Robert A Britton
Multiple GTPases participate in the assembly of the large ribosomal subunit in Bacillus subtilis.
J Bacteriol: 2006, 188(23);8252-8
[PubMed:16997968] [WorldCat.org] [DOI] (P p)

Sergey N Ruzheinikov, Sanjan K Das, Svetlana E Sedelnikova, Patrick J Baker, Peter J Artymiuk, Jorge García-Lara, Simon J Foster, David W Rice
Analysis of the open and closed conformations of the GTP-binding protein YsxC from Bacillus subtilis.
J Mol Biol: 2004, 339(2);265-78
[PubMed:15136032] [WorldCat.org] [DOI] (P p)

Sanjan K Das, Svetlana E Sedelnikova, Patrick J Baker, Sergey N Ruzheinikov, Simon J Foster, David W Rice
Expression, purification, crystallization and preliminary crystallographic analysis of a putative GTP-binding protein, YsxC, from Bacillus subtilis.
Acta Crystallogr D Biol Crystallogr: 2004, 60(Pt 1);166-8
[PubMed:14684918] [WorldCat.org] [DOI] (P p)

Takuya Morimoto, Pek Chin Loh, Tomohiro Hirai, Kei Asai, Kazuo Kobayashi, Shigeki Moriya, Naotake Ogasawara
Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis.
Microbiology (Reading): 2002, 148(Pt 11);3539-3552
[PubMed:12427945] [WorldCat.org] [DOI] (P p)

Z Prágai, C R Harwood
YsxC, a putative GTP-binding protein essential for growth of Bacillus subtilis 168.
J Bacteriol: 2000, 182(23);6819-23
[PubMed:11073929] [WorldCat.org] [DOI] (P p)

E Krüger, M Hecker
The first gene of the Bacillus subtilis clpC operon, ctsR, encodes a negative regulator of its own operon and other class III heat shock genes.
J Bacteriol: 1998, 180(24);6681-8
[PubMed:9852015] [WorldCat.org] [DOI] (P p)

S Riethdorf, U Völker, U Gerth, A Winkler, S Engelmann, M Hecker
Cloning, nucleotide sequence, and expression of the Bacillus subtilis lon gene.
J Bacteriol: 1994, 176(21);6518-27
[PubMed:7961402] [WorldCat.org] [DOI] (P p)