BofC

• Description: general stress protein, controls processing of pro-SigK by SpoIVFB
  
  

Gene name	bofC
Synonyms
Essential	no
Product	forespore regulator of the sigma-K checkpoint
Function	control of processing of pro-SigK by SpoIVFB
Gene expression levels in SubtiExpress: bofC
Interactions involving this protein in SubtInteract: BofC
MW, pI	19 kDa, 5.753
Gene length, protein length	510 bp, 170 aa
Immediate neighbours	ruvA, csbX
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sigma factors and their control, sporulation proteins, general stress proteins (controlled by SigB)

This gene is a member of the following regulons

SigB regulon, SigF regulon, SigG regulon

The gene

Basic information

• Locus tag: BSU27750

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • BofC-SpoIVB PubMed

• Localization:

Database entries

• Structure: 2BW2

• UniProt: O05391

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: csbX-bofC PubMed

• Expression browser: bofC PubMed

• Sigma factor: SigF PubMed, SigG PubMed, SigB PubMed

• Regulation:
  • expressed during sporulation in the forespore (SigF, SigG) PubMed
  • induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Hayley M Patterson, James A Brannigan, Simon M Cutting, Keith S Wilson, Anthony J Wilkinson, Eiso Ab, Tammo Diercks, Rob N de Jong, Vincent Truffault, Gert E Folkers, Robert Kaptein
The structure of bypass of forespore C, an intercompartmental signaling factor during sporulation in Bacillus.
J Biol Chem: 2005, 280(43);36214-20
[PubMed:16049010] [WorldCat.org] [DOI] (P p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)

P Wakeley, N T Hoa, S Cutting
BofC negatively regulates SpoIVB-mediated signalling in the Bacillus subtilis sigmaK-checkpoint.
Mol Microbiol: 2000, 36(6);1415-24
[PubMed:10931291] [WorldCat.org] [DOI] (P p)

M Gomez, S M Cutting
Identification of a new sigmaB-controlled gene, csbX, in Bacillus subtilis.
Gene: 1997, 188(1);29-33
[PubMed:9099855] [WorldCat.org] [DOI] (P p)

Manuel Gomez, Simon M Cutting
BofC encodes a putative forespore regulator of the Bacillus subtilis sigma K checkpoint.
Microbiology (Reading): 1997, 143 ( Pt 1);157-170
[PubMed:9025289] [WorldCat.org] [DOI] (P p)