XylR

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Gene name xylR
Synonyms
Essential no
Product transcriptional repressor
Function regulation of xylan and xylose utilization
Gene expression levels in SubtiExpress: xylR
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 38 kDa, 5.819
Gene length, protein length 1050 bp, 350 aa
Immediate neighbours xynB, xylA
Sequences Protein DNA Advanced_DNA
Genetic context
XylR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
XylR expression.png




























Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control

This gene is a member of the following regulons

The XylR regulon

The gene

Basic information

  • Locus tag: BSU17590

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transcription repression of the xylA-xylB and the xynP-xynB operons
  • Protein family: ROK (nagC/xylR) family (according to Swiss-Prot)
  • Paralogous protein(s): GlcK

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: inducer: xylose

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: xylR (according to DBTBS)
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP270 (erm), GP1302 (aphA3), available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Wolfgang Hillen, Erlangen University, Germany Homepage

Your additional remarks

References

M K Dahl, D Schmiedel, W Hillen
Glucose and glucose-6-phosphate interaction with Xyl repressor proteins from Bacillus spp. may contribute to regulation of xylose utilization.
J Bacteriol: 1995, 177(19);5467-72
[PubMed:7559331] [WorldCat.org] [DOI] (P p)

M K Dahl, J Degenkolb, W Hillen
Transcription of the xyl operon is controlled in Bacillus subtilis by tandem overlapping operators spaced by four base-pairs.
J Mol Biol: 1994, 243(3);413-24
[PubMed:7966270] [WorldCat.org] [DOI] (P p)

F Titgemeyer, J Reizer, A Reizer, M H Saier
Evolutionary relationships between sugar kinases and transcriptional repressors in bacteria.
Microbiology (Reading): 1994, 140 ( Pt 9);2349-54
[PubMed:7952186] [WorldCat.org] [DOI] (P p)

C Kauder, R Allmansberger, D Gärtner, D Schmiedel, W Hillen
An operator binding-negative mutation of Xyl repressor from Bacillus subtilis is trans dominant in Bacillus megaterium.
FEMS Microbiol Lett: 1993, 109(1);81-4
[PubMed:8319885] [WorldCat.org] [DOI] (P p)

D Gärtner, J Degenkolb, J A Ripperger, R Allmansberger, W Hillen
Regulation of the Bacillus subtilis W23 xylose utilization operon: interaction of the Xyl repressor with the xyl operator and the inducer xylose.
Mol Gen Genet: 1992, 232(3);415-22
[PubMed:1588910] [WorldCat.org] [DOI] (P p)

P Kreuzer, D Gärtner, R Allmansberger, W Hillen
Identification and sequence analysis of the Bacillus subtilis W23 xylR gene and xyl operator.
J Bacteriol: 1989, 171(7);3840-5
[PubMed:2544559] [WorldCat.org] [DOI] (P p)