YerD

• Description: general stress protein, similar to glutamate synthase (ferredoxin)
  
  

Gene name	yerD
Synonyms
Essential	no
Product	unknown
Function	unknown
Gene expression levels in SubtiExpress: yerD
MW, pI	58 kDa, 7.349
Gene length, protein length	1575 bp, 525 aa
Immediate neighbours	yerC, pcrB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

general stress proteins (controlled by SigB), poorly characterized/ putative enzymes, membrane proteins

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

• Locus tag: BSU06590

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): GltA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to UniProt)

Database entries

• Structure:

• UniProt: O34849

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yerD PubMed

• Expression browser: yerD PubMed

• Sigma factor: SigB PubMed

• Regulation: induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP1184 (tet) available in Jörg Stülke's lab

• Expression vector: pBP32 (expression vector for E. coli BL21; N-terminal Strep-tag-YerD fusion protein, based on pGP172), available in Fabian Commichau's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• FLAG-tag construct: GP1187 (spc, based on pGP1331), available in Jörg Stülke's lab

• Antibody:

References

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)