NagR

• Description: transcriptional regulator (GntR family)
  
  

Gene name	nagR
Synonyms	yvoA
Essential	no
Product	transcriptional regulator (GntR family)
Function	probably regulator of the putative nagA-nagB-yvoA operon
Gene expression levels in SubtiExpress: nagR
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism, Murein recycling
MW, pI	27 kDa, 6.768
Gene length, protein length	729 bp, 243 aa
Immediate neighbours	nagB, yvnB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control

This gene is a member of the following regulons

NagR regulon

The NagR regulon:

• nagA-nagB-nagR, nagP

The gene

Basic information

• Locus tag: BSU35030

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription repression of nagP and of the putative nagA-nagB-nagR operon in the absence of N-acetylglycosamine PubMed, weak repression of yflG PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: K(D) for N-acetylglucosamine-6-phosphate: 1 mM PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • N-acetylglucosamine-6-phosphate acts as the molecular inducer PubMed
  • glucosamine-6-phosphate acts as the molecular inducerPubMed

• Interactions:

• Localization:

Database entries

• Structure: 2WVO PubMed

• UniProt: O34817

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: nagA-nagB-nagR PubMed

• Expression browser: nagR PubMed

• Sigma factor:

• Regulation:
  • induced in the presence of N-acetylglucosamine (NagR) PubMed

• Regulatory mechanism:
  • NagR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Marcus Resch, Emile Schiltz, Fritz Titgemeyer, Yves A Muller
Insight into the induction mechanism of the GntR/HutC bacterial transcription regulator YvoA.
Nucleic Acids Res: 2010, 38(7);2485-97
[PubMed:20047956] [WorldCat.org] [DOI] (I p)

Marcus Resch, Heide Marie Roth, Mathias Kottmair, Madhumati Sevvana, Ralph Bertram, Fritz Titgemeyer, Yves A Muller
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of YvoA from Bacillus subtilis.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2009, 65(Pt 4);410-4
[PubMed:19342794] [WorldCat.org] [DOI] (I p)

CongHui You, HongYan Lu, Agnieszka Sekowska, Gang Fang, YiPing Wang, Anne-Marie Gilles, Antoine Danchin
The two authentic methionine aminopeptidase genes are differentially expressed in Bacillus subtilis.
BMC Microbiol: 2005, 5;57
[PubMed:16207374] [WorldCat.org] [DOI] (I e)