TrmB

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  • Description: tRNA (m7G46) methyltransferase

Gene name trmB
Synonyms ytmQ
Essential no
Product tRNA (m7G46) methyltransferase
Function tRNA modification
Gene expression levels in SubtiExpress: trmB
MW, pI 24 kDa, 6.351
Gene length, protein length 639 bp, 213 aa
Immediate neighbours ytnP, ytzH
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YtmQ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TrmB expression.png
























Categories containing this gene/protein

translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU29900

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine (according to Swiss-Prot)
  • Protein family: TrmB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ingrid Zegers, Daniel Gigot, Françoise van Vliet, Catherine Tricot, Stéphane Aymerich, Janusz M Bujnicki, Jan Kosinski, Louis Droogmans
Crystal structure of Bacillus subtilis TrmB, the tRNA (m7G46) methyltransferase.
Nucleic Acids Res: 2006, 34(6);1925-34
[PubMed:16600901] [WorldCat.org] [DOI] (I e)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)