SppA

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  • Description: signal peptide peptidase required for efficient processing of pre-proteins

Gene name sppA
Synonyms yteI
Essential no
Product signal peptide peptidase
Function protein secretion
Gene expression levels in SubtiExpress: sppA
Metabolic function and regulation of this protein in SubtiPathways:
Protein secretion
MW, pI 36 kDa, 7.314
Gene length, protein length 1005 bp, 335 aa
Immediate neighbours yteJ, ytdI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SppA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SppA expression.png
























Categories containing this gene/protein

protein secretion, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigW regulon

The gene

Basic information

  • Locus tag: BSU29530

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • serine protease that functions to cleave the remnant signal peptides left behind after protein secretion and cleavage by signal peptidases
  • Protein family: peptidase S49 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced in response to cell wall stress (SigW) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Sung-Eun Nam, Apollos C Kim, Mark Paetzel
Crystal structure of Bacillus subtilis signal peptide peptidase A.
J Mol Biol: 2012, 419(5);347-58
[PubMed:22472423] [WorldCat.org] [DOI] (I p)

Min Cao, Tao Wang, Rick Ye, John D Helmann
Antibiotics that inhibit cell wall biosynthesis induce expression of the Bacillus subtilis sigma(W) and sigma(M) regulons.
Mol Microbiol: 2002, 45(5);1267-76
[PubMed:12207695] [WorldCat.org] [DOI] (P p)

X Huang, A Gaballa, M Cao, J D Helmann
Identification of target promoters for the Bacillus subtilis extracytoplasmic function sigma factor, sigma W.
Mol Microbiol: 1999, 31(1);361-71
[PubMed:9987136] [WorldCat.org] [DOI] (P p)