AbnA

• Description: endo-1,5-alpha-L-arabinosidase
  
  

Gene name	abnA
Synonyms
Essential	no
Product	endo-1,5-alpha-L-arabinosidase
Function	arabinan degradation
Gene expression levels in SubtiExpress: abnA
MW, pI	34 kDa, 8.618
Gene length, protein length	939 bp, 313 aa
Immediate neighbours	araA, ysdC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

AraR regulon

The gene

Basic information

• Locus tag: BSU28810

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endohydrolysis of (1->5)-alpha-arabinofuranosidic linkages in (1->5)-arabinans (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 43 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide) PubMed

Database entries

• Structure: 1UV4

• UniProt: P94522

• KEGG entry: [3]

• E.C. number: 3.2.1.99

Expression and regulation

• Operon: abnA PubMed

• Expression browser: abnA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the absence of arabinose (AraR) PubMed

• Regulatory mechanism:
  • AraR: transcription repression PubMed

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Teresa Fontes Leal, Isabel de Sá-Nogueira
Purification, characterization and functional analysis of an endo-arabinanase (AbnA) from Bacillus subtilis.
FEMS Microbiol Lett: 2004, 241(1);41-8
[PubMed:15556708] [WorldCat.org] [DOI] (P p)

Maria Paiva Raposo, José Manuel Inácio, Luís Jaime Mota, Isabel de Sá-Nogueira
Transcriptional regulation of genes encoding arabinan-degrading enzymes in Bacillus subtilis.
J Bacteriol: 2004, 186(5);1287-96
[PubMed:14973026] [WorldCat.org] [DOI] (P p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)