YqjM

• Description: NADPH-dependent flavin oxidoreductase
  
  

Gene name	yqjM
Synonyms
Essential	no
Product	NADPH-dependent flavin oxidoreductase
Function	reduction of double bonds of nonsaturated aldehydes and ketones, may be involved in detoxification
Gene expression levels in SubtiExpress: yqjM ketones, may be involved in detoxification
MW, pI	37 kDa, 5.137
Gene length, protein length	1014 bp, 338 aa
Immediate neighbours	yqjN, yqjL
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

electron transport/ other, resistance against oxidative and electrophile stress

The gene

Basic information

• Locus tag: BSU23820

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: NADPH + acceptor = NADP⁺ + reduced acceptor (according to Swiss-Prot)

• Protein family: NamA subfamily (according to Swiss-Prot)

• Paralogous protein(s): YqiG

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1Z41 (oxidized form), 1Z48 (reduced form)

• UniProt: P54550

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yqjM (according to DBTBS)

• Expression browser: yqjM PubMed

• Sigma factor:

• Regulation:
  • induced in the presence of H(2)O(2) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Karina Kitzing, Teresa B Fitzpatrick, Corinna Wilken, Justyna Sawa, Gleb P Bourenkov, Peter Macheroux, Tim Clausen
The 1.3 A crystal structure of the flavoprotein YqjM reveals a novel class of Old Yellow Enzymes.
J Biol Chem: 2005, 280(30);27904-13
[PubMed:15890652] [WorldCat.org] [DOI] (P p)

Teresa B Fitzpatrick, Sigrid Auweter, Karina Kitzing, Tim Clausen, Nikolaus Amrhein, Peter Macheroux
Structural and functional impairment of an Old Yellow Enzyme homologue upon affinity tag incorporation.
Protein Expr Purif: 2004, 36(2);280-91
[PubMed:15249051] [WorldCat.org] [DOI] (P p)

Teresa B Fitzpatrick, Nikolaus Amrhein, Peter Macheroux
Characterization of YqjM, an Old Yellow Enzyme homolog from Bacillus subtilis involved in the oxidative stress response.
J Biol Chem: 2003, 278(22);19891-7
[PubMed:12660247] [WorldCat.org] [DOI] (P p)