PrfB

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  • Description: peptide chain release factor 2

Gene name prfB
Synonyms
Essential yes PubMed
Product peptide chain release factor 2
Function translation
Metabolic function and regulation of this protein in SubtiPathways:
Protein secretion
MW, pI 41 kDa, 4.85
Gene length, protein length 1099 bp, 366 aa
Immediate neighbours yvjA, secA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PrfB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PrfB expression.png
























Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU35290

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: binds to the ribosome through specific recognition of messenger RNA stop codons and triggers hydrolysis of the bond between the nascent polypeptide and the transfer RNA at the peptidyl-tRNA site, thereby releasing the newly synthesized protein. PrfB is highly specific for a U in the first stop-codon position and recognizes UAA and UGA
  • Protein family: prokaryotic/mitochondrial release factor family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Johan Sund, Martin Andér, Johan Aqvist
Principles of stop-codon reading on the ribosome.
Nature: 2010, 465(7300);947-50
[PubMed:20512119] [WorldCat.org] [DOI] (I p)

M Herbort, M Klein, E H Manting, A J Driessen, R Freudl
Temporal expression of the Bacillus subtilis secA gene, encoding a central component of the preprotein translocase.
J Bacteriol: 1999, 181(2);493-500
[PubMed:9882663] [WorldCat.org] [DOI] (P p)