NucB

• Description: sporulation-specific extracellular nuclease
  
  

Gene name	nucB
Synonyms
Essential	no
Product	sporulation-specific extracellular nuclease
Function	DNA degradation after mother cell lysis
Gene expression levels in SubtiExpress: nucB
MW, pI	14 kDa, 5.173
Gene length, protein length	408 bp, 136 aa
Immediate neighbours	yqeB, spoIVCB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of nucleotides, membrane proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

• Locus tag: BSU25750

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P42983

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nucB PubMed

• Expression browser: nucB PubMed

• Sigma factor: SigE PubMed

• Regulation: expressed during sporulation (SigE) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Shigeo Hosoya, Zuolei Lu, Yousuke Ozaki, Michio Takeuchi, Tsutomu Sato
Cytological analysis of the mother cell death process during sporulation in Bacillus subtilis.
J Bacteriol: 2007, 189(6);2561-5
[PubMed:17209033] [WorldCat.org] [DOI] (P p)

D van Sinderen, R Kiewiet, G Venema
Differential expression of two closely related deoxyribonuclease genes, nucA and nucB, in Bacillus subtilis.
Mol Microbiol: 1995, 15(2);213-23
[PubMed:7746143] [WorldCat.org] [DOI] (P p)