MraY

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  • Description: phospho-N-acetylmuramoyl-pentapeptide-transferase (meso-2,6-diaminopimelate), catalyzes the first commited membrane-bound step of bacterial peptidoglycan synthesis leading to the formation of lipid I

Gene name mraY
Synonyms
Essential yes PubMed
Product phospho-N-acetylmuramoyl-pentapeptide-transferase
(meso-2,6-diaminopimelate)
Function peptidoglycan precursor biosynthesis
Metabolic function and regulation of this protein in SubtiPathways:
Cell wall
MW, pI 35 kDa, 8.966
Gene length, protein length 972 bp, 324 aa
Immediate neighbours murE, murD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MraY context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MraY expression.png




























Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU15190

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol (according to Swiss-Prot)
  • Protein family: MraY subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • expressed during vegatative growth PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Yi Ma, Daniela Münch, Tanja Schneider, Hans-Georg Sahl, Ahmed Bouhss, Umesh Ghoshdastider, Jufang Wang, Volker Dötsch, Xiaoning Wang, Frank Bernhard
Preparative scale cell-free production and quality optimization of MraY homologues in different expression modes.
J Biol Chem: 2011, 286(45);38844-53
[PubMed:21937437] [WorldCat.org] [DOI] (I p)

Delphine Lecerclé, Anthony Clouet, Bayan Al-Dabbagh, Muriel Crouvoisier, Ahmed Bouhss, Christine Gravier-Pelletier, Yves Le Merrer
Bacterial transferase MraY inhibitors: synthesis and biological evaluation.
Bioorg Med Chem: 2010, 18(12);4560-9
[PubMed:20537545] [WorldCat.org] [DOI] (I p)

Yi Zheng, Douglas K Struck, Thomas G Bernhardt, Ry Young
Genetic analysis of MraY inhibition by the phiX174 protein E.
Genetics: 2008, 180(3);1459-66
[PubMed:18791230] [WorldCat.org] [DOI] (P p)

Bayan Al-Dabbagh, Xavier Henry, Meriem El Ghachi, Geneviève Auger, Didier Blanot, Claudine Parquet, Dominique Mengin-Lecreulx, Ahmed Bouhss
Active site mapping of MraY, a member of the polyprenyl-phosphate N-acetylhexosamine 1-phosphate transferase superfamily, catalyzing the first membrane step of peptidoglycan biosynthesis.
Biochemistry: 2008, 47(34);8919-28
[PubMed:18672909] [WorldCat.org] [DOI] (I p)

Ahmed Bouhss, Amy E Trunkfield, Timothy D H Bugg, Dominique Mengin-Lecreulx
The biosynthesis of peptidoglycan lipid-linked intermediates.
FEMS Microbiol Rev: 2008, 32(2);208-33
[PubMed:18081839] [WorldCat.org] [DOI] (P p)

Ahmed Bouhss, Muriel Crouvoisier, Didier Blanot, Dominique Mengin-Lecreulx
Purification and characterization of the bacterial MraY translocase catalyzing the first membrane step of peptidoglycan biosynthesis.
J Biol Chem: 2004, 279(29);29974-80
[PubMed:15131133] [WorldCat.org] [DOI] (P p)

A A Branstrom, S Midha, C B Longley, K Han, E R Baizman, H R Axelrod
Assay for identification of inhibitors for bacterial MraY translocase or MurG transferase.
Anal Biochem: 2000, 280(2);315-9
[PubMed:10790316] [WorldCat.org] [DOI] (P p)

M A Lehrman
A family of UDP-GlcNAc/MurNAc: polyisoprenol-P GlcNAc/MurNAc-1-P transferases.
Glycobiology: 1994, 4(6);768-71
[PubMed:7734839] [WorldCat.org] [DOI] (P p)

R A Daniel, J Errington
DNA sequence of the murE-murD region of Bacillus subtilis 168.
J Gen Microbiol: 1993, 139(2);361-70
[PubMed:8436954] [WorldCat.org] [DOI] (P p)

A O Henriques, H de Lencastre, P J Piggot
A Bacillus subtilis morphogene cluster that includes spoVE is homologous to the mra region of Escherichia coli.
Biochimie: 1992, 74(7-8);735-48
[PubMed:1391053] [WorldCat.org] [DOI] (P p)