TuaA/2

• Description: lipid carrier sugar transferase, disrupted pseudogene in B. subtilis 168
  
  

Gene name	tuaA
Synonyms	yvhA
Essential	no
Product	lipid carrier sugar transferase
Function	biosynthesis of teichuronic acid
MW, pI	- , -
Gene length, protein length	486 bp, -
Immediate neighbours	tuaA/1, lytC
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, membrane proteins, pseudogenes

This gene is a member of the following regulons

PhoP regulon

The gene

Basic information

• Locus tag: BSU35610

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: UDP-N-acetyl-D-galactosamine + undecaprenyl phosphate = UMP + N-acetyl-alpha-D-galactosaminyldiphospho-undecaprenol (according to Swiss-Prot)

• Protein family: bacterial sugar transferase family (according to Swiss-Prot)

• Paralogous protein(s): EpsL

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: O32274

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: tuaA-tuaB-tuaC-tuaD-tuaE-tuaF-tuaG-tuaH PubMed

• Expression browser: tuaA/2 PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Maryam Lahooti, Colin R Harwood
Transcriptional analysis of the Bacillus subtilis teichuronic acid operon.
Microbiology (Reading): 1999, 145 ( Pt 12);3409-3417
[PubMed:10627039] [WorldCat.org] [DOI] (P p)

B Soldo, V Lazarevic, M Pagni, D Karamata
Teichuronic acid operon of Bacillus subtilis 168.
Mol Microbiol: 1999, 31(3);795-805
[PubMed:10048024] [WorldCat.org] [DOI] (P p)

Y Qi, F M Hulett
Role of Pho-P in transcriptional regulation of genes involved in cell wall anionic polymer biosynthesis in Bacillus subtilis.
J Bacteriol: 1998, 180(15);4007-10
[PubMed:9683503] [WorldCat.org] [DOI] (P p)

Wei Liu, F Marion Hulett
Comparison of PhoP binding to the tuaA promoter with PhoP binding to other Pho-regulon promoters establishes a Bacillus subtilis Pho core binding site.
Microbiology (Reading): 1998, 144 ( Pt 5);1443-1450
[PubMed:9611818] [WorldCat.org] [DOI] (P p)