BshB1

• Description: N-acetylglucosamin-malate deacetylase, involved in bacillithiol synthesis
  
  

Gene name	bshB1
Synonyms	jojG, ypjG
Essential	no
Product	N-acetylglucosamin-malate deacetylase
Function	biosynthesis of bacillithiol
Metabolic function and regulation of this protein in SubtiPathways: Lys, Thr
MW, pI	24 kDa, 6.343
Gene length, protein length	672 bp, 224 aa
Immediate neighbours	bshA, mgsA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

miscellaneous metabolic pathways, resistance against oxidative and electrophile stress

The gene

Basic information

• Locus tag: BSU22470

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: GlcNAc-Mal -> GlcN-Mal + Ac

• Protein family:

• Paralogous protein(s): BshB2

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P42981

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: ypjD-dapB-mgsA-bshB1-bshA-cca-birA PubMed

• Expression browser: bshB1 PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: bshB1 null mutant and bshB1 / bshB2 double null mutant available in John Helmann lab
  • GP87 (ypjG::pX2(cat)), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)

Gerald L Newton, Mamta Rawat, James J La Clair, Vishnu Karthik Jothivasan, Tanya Budiarto, Chris J Hamilton, Al Claiborne, John D Helmann, Robert C Fahey
Bacillithiol is an antioxidant thiol produced in Bacilli.
Nat Chem Biol: 2009, 5(9);625-7
[PubMed:19578333] [WorldCat.org] [DOI] (I p)