QueA

• Description: S-adenosylmethionine tRNA ribosyltransferase
  
  

Gene name	queA
Synonyms
Essential	no
Product	S-adenosylmethionine tRNA ribosyltransferase
Function	tRNA modification
MW, pI	38 kDa, 5.047
Gene length, protein length	1026 bp, 342 aa
Immediate neighbours	tgt, ruvB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

translation

The gene

Basic information

• Locus tag: BSU27720

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: S-adenosylmethionine + 7-aminomethyl-7-deazaguanosine = methionine + adenine + epoxyqueuosine (according to Swiss-Prot)

• Protein family: queA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1YY3

• UniProt: O32054

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: queA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Clemens Grimm, Ralf Ficner, Tanja Sgraja, Peter Haebel, Gerhard Klebe, Klaus Reuter
Crystal structure of Bacillus subtilis S-adenosylmethionine:tRNA ribosyltransferase-isomerase.
Biochem Biophys Res Commun: 2006, 351(3);695-701
[PubMed:17083917] [WorldCat.org] [DOI] (P p)

C Grimm, G Klebe, R Ficner, K Reuter
Screening orthologs as an important variable in crystallization: preliminary X-ray diffraction studies of the tRNA-modifying enzyme S-adenosyl-methionine:tRNA ribosyl transferase/isomerase.
Acta Crystallogr D Biol Crystallogr: 2000, 56(Pt 4);484-8
[PubMed:10739928] [WorldCat.org] [DOI] (P p)