PhoD

• Description: phosphodiesterase/alkaline phosphatase
  
  

Gene name	phoD
Synonyms	ycbS
Essential	no
Product	phosphodiesterase/alkaline phosphatase
Function	aquisition of phosphate upon phosphoate starvation
Interactions involving this protein in SubtInteract: PhoD
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	62 kDa, 8.394
Gene length, protein length	1668 bp, 556 aa
Immediate neighbours	ycbR, tatAD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

phosphate metabolism

This gene is a member of the following regulons

PhoP regulon

The gene

Basic information

• Locus tag: BSU02620

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: A phosphate monoester + H₂O = an alcohol + phosphate (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • TatAD-PhoD PubMed

• Localization: extracellular (signal peptide) PubMed, secreted by the TatAD-TatCD complex PubMed

Database entries

• Structure:

• UniProt: P42251

• KEGG entry: [3]

• E.C. number: 3.1.3.1

Expression and regulation

• Operon: phoD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Oscar Kuipers, University of Groningen, The Netherlands Homepage